Construct: shRNA TRCN0000371148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001127212.1-225s21c1
DNA Barcode:
GGGAACCAGCAACTCAACGTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE2A (729447)
Gene Description:
G antigen 2A
Transcript:
RefSeq NM_001127212.1 (CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAACCAGCAACTCAACGTC
Hairpin Sequence:
5'-CCGG-GGGAACCAGCAACTCAACGTC-CTCGAG-GACGTTGAGTTGCTGGTTCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAACCAGCAACTCAACGTCCTCGAGGACGTTGAGTTGCTGGTTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAACCAGCAACTCAACGTCCTCGAGGACGTTGAGTTGCTGGTTCCC-3'

Other clones with same target sequence:

(none)