Construct: shRNA TRCN0000371153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145784.1-1188s21c1
DNA Barcode:
TCCCTTGTCCTAGGTTCCTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BORCS8 (729991)
Gene Description:
BLOC-1 related complex subunit 8
Transcript:
RefSeq NM_001145784.1 (NON-CURRENT)
Match location:
Position 1188 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCTTGTCCTAGGTTCCTAG
Hairpin Sequence:
5'-CCGG-TCCCTTGTCCTAGGTTCCTAG-CTCGAG-CTAGGAACCTAGGACAAGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCTTGTCCTAGGTTCCTAGCTCGAGCTAGGAACCTAGGACAAGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCTTGTCCTAGGTTCCTAGCTCGAGCTAGGAACCTAGGACAAGGGA-3'

Other clones with same target sequence:

(none)