Construct: shRNA TRCN0000371239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176813.3-249s21c1
DNA Barcode:
AGAAGCCATTAATGGTTATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGR3 (155465)
Gene Description:
anterior gradient 3, protein disulphide isomerase family member
Transcript:
RefSeq NM_176813.3 (NON-CURRENT)
Match location:
Position 249 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGCCATTAATGGTTATTC
Hairpin Sequence:
5'-CCGG-AGAAGCCATTAATGGTTATTC-CTCGAG-GAATAACCATTAATGGCTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGCCATTAATGGTTATTCCTCGAGGAATAACCATTAATGGCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGCCATTAATGGTTATTCCTCGAGGAATAACCATTAATGGCTTCT-3'

Other clones with same target sequence:

(none)