Construct: shRNA TRCN0000371301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001633.3-1020s21c1
DNA Barcode:
TCCATGGCCTGTGAGACTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMBP (259)
Gene Description:
alpha-1-microglobulin/bikunin precursor
Transcript:
RefSeq NM_001633.3 (NON-CURRENT)
Match location:
Position 1020 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCATGGCCTGTGAGACTTTC
Hairpin Sequence:
5'-CCGG-TCCATGGCCTGTGAGACTTTC-CTCGAG-GAAAGTCTCACAGGCCATGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCATGGCCTGTGAGACTTTCCTCGAGGAAAGTCTCACAGGCCATGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCATGGCCTGTGAGACTTTCCTCGAGGAAAGTCTCACAGGCCATGGA-3'

Other clones with same target sequence:

(none)