Construct: shRNA TRCN0000371432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133468.3-1049s21c1
DNA Barcode:
AGATGTGGTCCTCTATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMPER (168667)
Gene Description:
BMP binding endothelial regulator
Transcript:
RefSeq NM_133468.3 (NON-CURRENT)
Match location:
Position 1049 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATGTGGTCCTCTATCAATT
Hairpin Sequence:
5'-CCGG-AGATGTGGTCCTCTATCAATT-CTCGAG-AATTGATAGAGGACCACATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATGTGGTCCTCTATCAATTCTCGAGAATTGATAGAGGACCACATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATGTGGTCCTCTATCAATTCTCGAGAATTGATAGAGGACCACATCT-3'

Other clones with same target sequence:

(none)