Construct: shRNA TRCN0000371583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024821.2-637s21c1
DNA Barcode:
CAACTTCCAGAACCCATTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC134 (79879)
Gene Description:
coiled-coil domain containing 134
Transcript:
RefSeq NM_024821.2 (NON-CURRENT)
Match location:
Position 637 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTTCCAGAACCCATTTAA
Hairpin Sequence:
5'-CCGG-CAACTTCCAGAACCCATTTAA-CTCGAG-TTAAATGGGTTCTGGAAGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTTCCAGAACCCATTTAACTCGAGTTAAATGGGTTCTGGAAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTTCCAGAACCCATTTAACTCGAGTTAAATGGGTTCTGGAAGTTG-3'

Other clones with same target sequence:

(none)