Construct: shRNA TRCN0000371754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021155.3-1666s21c1
DNA Barcode:
ACTGGTTGCAAGAGCTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD209 (30835)
Gene Description:
CD209 molecule
Transcript:
RefSeq NM_021155.3 (NON-CURRENT)
Match location:
Position 1666 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGTTGCAAGAGCTCATTT
Hairpin Sequence:
5'-CCGG-ACTGGTTGCAAGAGCTCATTT-CTCGAG-AAATGAGCTCTTGCAACCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGTTGCAAGAGCTCATTTCTCGAGAAATGAGCTCTTGCAACCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGTTGCAAGAGCTCATTTCTCGAGAAATGAGCTCTTGCAACCAGT-3'

Other clones with same target sequence:

(none)