Construct: shRNA TRCN0000371772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024922.4-444s21c1
DNA Barcode:
GCCTGGTCCTCAACGTCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CES3 (23491)
Gene Description:
carboxylesterase 3
Transcript:
RefSeq NM_024922.4 (NON-CURRENT)
Match location:
Position 444 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGTCCTCAACGTCTATA
Hairpin Sequence:
5'-CCGG-GCCTGGTCCTCAACGTCTATA-CTCGAG-TATAGACGTTGAGGACCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGTCCTCAACGTCTATACTCGAGTATAGACGTTGAGGACCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGTCCTCAACGTCTATACTCGAGTATAGACGTTGAGGACCAGGC-3'

Other clones with same target sequence:

(none)