Construct: shRNA TRCN0000371778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030787.3-1094s21c1
DNA Barcode:
TGTTGCAACACACCAACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFHR5 (81494)
Gene Description:
complement factor H related 5
Transcript:
RefSeq NM_030787.3 (NON-CURRENT)
Match location:
Position 1094 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGCAACACACCAACTTAA
Hairpin Sequence:
5'-CCGG-TGTTGCAACACACCAACTTAA-CTCGAG-TTAAGTTGGTGTGTTGCAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGCAACACACCAACTTAACTCGAGTTAAGTTGGTGTGTTGCAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGCAACACACCAACTTAACTCGAGTTAAGTTGGTGTGTTGCAACA-3'

Other clones with same target sequence:

(none)