Construct: shRNA TRCN0000371782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006569.4-613s21c1
DNA Barcode:
TGATCTTGATAGTGGACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CGREF1 (10669)
Gene Description:
cell growth regulator with EF-hand domain 1
Transcript:
RefSeq NM_006569.4 (NON-CURRENT)
Match location:
Position 613 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCTTGATAGTGGACAAAG
Hairpin Sequence:
5'-CCGG-TGATCTTGATAGTGGACAAAG-CTCGAG-CTTTGTCCACTATCAAGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCTTGATAGTGGACAAAGCTCGAGCTTTGTCCACTATCAAGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCTTGATAGTGGACAAAGCTCGAGCTTTGTCCACTATCAAGATCA-3'

Other clones with same target sequence:

(none)