Construct: shRNA TRCN0000371784

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001276.2-774s21c1
DNA Barcode:
TAGCATCATGACCTACGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHI3L1 (1116)
Gene Description:
chitinase 3 like 1
Transcript:
RefSeq NM_001276.2 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371784)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCATCATGACCTACGATTT
Hairpin Sequence:
5'-CCGG-TAGCATCATGACCTACGATTT-CTCGAG-AAATCGTAGGTCATGATGCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCATCATGACCTACGATTTCTCGAGAAATCGTAGGTCATGATGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCATCATGACCTACGATTTCTCGAGAAATCGTAGGTCATGATGCTA-3'

Other clones with same target sequence:

(none)