Construct: shRNA TRCN0000371850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031461.4-2185s21c1
DNA Barcode:
CTTTGTATATGCCACTAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRISPLD1 (83690)
Gene Description:
cysteine rich secretory protein LCCL domain containing 1
Transcript:
RefSeq NM_031461.4 (NON-CURRENT)
Match location:
Position 2185 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGTATATGCCACTAATAA
Hairpin Sequence:
5'-CCGG-CTTTGTATATGCCACTAATAA-CTCGAG-TTATTAGTGGCATATACAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGTATATGCCACTAATAACTCGAGTTATTAGTGGCATATACAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGTATATGCCACTAATAACTCGAGTTATTAGTGGCATATACAAAG-3'

Other clones with same target sequence:

(none)