Construct: shRNA TRCN0000371868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030820.3-1951s21c1
DNA Barcode:
ACATGGAAAGGATGGATTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL21A1 (81578)
Gene Description:
collagen type XXI alpha 1 chain
Transcript:
RefSeq NM_030820.3 (NON-CURRENT)
Match location:
Position 1951 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATGGAAAGGATGGATTAAT
Hairpin Sequence:
5'-CCGG-ACATGGAAAGGATGGATTAAT-CTCGAG-ATTAATCCATCCTTTCCATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATGGAAAGGATGGATTAATCTCGAGATTAATCCATCCTTTCCATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATGGAAAGGATGGATTAATCTCGAGATTAATCCATCCTTTCCATGT-3'

Other clones with same target sequence:

(none)