Construct: shRNA TRCN0000371878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005202.1-2237s21c1
DNA Barcode:
TTGGCCCGAGGAGACTAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL8A2 (1296)
Gene Description:
collagen type VIII alpha 2 chain
Transcript:
RefSeq NM_005202.1 (NON-CURRENT)
Match location:
Position 2237 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGCCCGAGGAGACTAACTT
Hairpin Sequence:
5'-CCGG-TTGGCCCGAGGAGACTAACTT-CTCGAG-AAGTTAGTCTCCTCGGGCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGCCCGAGGAGACTAACTTCTCGAGAAGTTAGTCTCCTCGGGCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGCCCGAGGAGACTAACTTCTCGAGAAGTTAGTCTCCTCGGGCCAA-3'

Other clones with same target sequence:

(none)