Construct: shRNA TRCN0000371901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052854.2-2168s21c1
DNA Barcode:
CCGACCACTCTGTTCTCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CREB3L1 (90993)
Gene Description:
cAMP responsive element binding protein 3 like 1
Transcript:
RefSeq NM_052854.2 (NON-CURRENT)
Match location:
Position 2168 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACCACTCTGTTCTCATTC
Hairpin Sequence:
5'-CCGG-CCGACCACTCTGTTCTCATTC-CTCGAG-GAATGAGAACAGAGTGGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACCACTCTGTTCTCATTCCTCGAGGAATGAGAACAGAGTGGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACCACTCTGTTCTCATTCCTCGAGGAATGAGAACAGAGTGGTCGG-3'

Other clones with same target sequence:

(none)