Construct: shRNA TRCN0000371912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031476.3-1201s21c1
DNA Barcode:
ATGAAAGCTCGTCTAGCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRISPLD2 (83716)
Gene Description:
cysteine rich secretory protein LCCL domain containing 2
Transcript:
RefSeq NM_031476.3 (NON-CURRENT)
Match location:
Position 1201 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAAAGCTCGTCTAGCATAT
Hairpin Sequence:
5'-CCGG-ATGAAAGCTCGTCTAGCATAT-CTCGAG-ATATGCTAGACGAGCTTTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAAAGCTCGTCTAGCATATCTCGAGATATGCTAGACGAGCTTTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAAAGCTCGTCTAGCATATCTCGAGATATGCTAGACGAGCTTTCAT-3'

Other clones with same target sequence:

(none)