Construct: shRNA TRCN0000371929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005212.2-254s21c1
DNA Barcode:
AGACCAGCTATAGCAATTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSN3 (1448)
Gene Description:
casein kappa
Transcript:
RefSeq NM_005212.2 (CURRENT)
Match location:
Position 254 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCAGCTATAGCAATTAAT
Hairpin Sequence:
5'-CCGG-AGACCAGCTATAGCAATTAAT-CTCGAG-ATTAATTGCTATAGCTGGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCAGCTATAGCAATTAATCTCGAGATTAATTGCTATAGCTGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCAGCTATAGCAATTAATCTCGAGATTAATTGCTATAGCTGGTCT-3'

Other clones with same target sequence:

(none)