Construct: shRNA TRCN0000372013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004887.4-851s21c1
DNA Barcode:
CAAAGGACTTTGCAGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CXCL14 (9547)
Gene Description:
C-X-C motif chemokine ligand 14
Transcript:
RefSeq NM_004887.4 (NON-CURRENT)
Match location:
Position 851 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGGACTTTGCAGATTAAA
Hairpin Sequence:
5'-CCGG-CAAAGGACTTTGCAGATTAAA-CTCGAG-TTTAATCTGCAAAGTCCTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGGACTTTGCAGATTAAACTCGAGTTTAATCTGCAAAGTCCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGGACTTTGCAGATTAAACTCGAGTTTAATCTGCAAAGTCCTTTG-3'

Other clones with same target sequence:

(none)