Construct: shRNA TRCN0000372047

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001935.3-1031s21c1
DNA Barcode:
GGTCACCAGTGGGTCATAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPP4 (1803)
Gene Description:
dipeptidyl peptidase 4
Transcript:
RefSeq NM_001935.3 (NON-CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372047)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCACCAGTGGGTCATAAAT
Hairpin Sequence:
5'-CCGG-GGTCACCAGTGGGTCATAAAT-CTCGAG-ATTTATGACCCACTGGTGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCACCAGTGGGTCATAAATCTCGAGATTTATGACCCACTGGTGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCACCAGTGGGTCATAAATCTCGAGATTTATGACCCACTGGTGACC-3'

Other clones with same target sequence:

(none)