Construct: shRNA TRCN0000372058

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016215.3-1120s21c1
DNA Barcode:
TGCAAGAAAGACTCGTGACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGFL7 (51162)
Gene Description:
EGF like domain multiple 7
Transcript:
RefSeq NM_016215.3 (NON-CURRENT)
Match location:
Position 1120 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372058)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAAGAAAGACTCGTGACTG
Hairpin Sequence:
5'-CCGG-TGCAAGAAAGACTCGTGACTG-CTCGAG-CAGTCACGAGTCTTTCTTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAAGAAAGACTCGTGACTGCTCGAGCAGTCACGAGTCTTTCTTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAAGAAAGACTCGTGACTGCTCGAGCAGTCACGAGTCTTTCTTGCA-3'

Other clones with same target sequence:

(none)