Construct: shRNA TRCN0000372137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000799.2-936s21c1
DNA Barcode:
AGAGCAACTCTGAGATCTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPO (2056)
Gene Description:
erythropoietin
Transcript:
RefSeq NM_000799.2 (NON-CURRENT)
Match location:
Position 936 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCAACTCTGAGATCTAAG
Hairpin Sequence:
5'-CCGG-AGAGCAACTCTGAGATCTAAG-CTCGAG-CTTAGATCTCAGAGTTGCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCAACTCTGAGATCTAAGCTCGAGCTTAGATCTCAGAGTTGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCAACTCTGAGATCTAAGCTCGAGCTTAGATCTCAGAGTTGCTCT-3'

Other clones with same target sequence:

(none)