Construct: shRNA TRCN0000372258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020638.2-1184s21c1
DNA Barcode:
GGGTCTCTCCCAACATATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGF23 (8074)
Gene Description:
fibroblast growth factor 23
Transcript:
RefSeq NM_020638.2 (NON-CURRENT)
Match location:
Position 1184 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTCTCTCCCAACATATTTC
Hairpin Sequence:
5'-CCGG-GGGTCTCTCCCAACATATTTC-CTCGAG-GAAATATGTTGGGAGAGACCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTCTCTCCCAACATATTTCCTCGAGGAAATATGTTGGGAGAGACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTCTCTCCCAACATATTTCCTCGAGGAAATATGTTGGGAGAGACCC-3'

Other clones with same target sequence:

(none)