Construct: shRNA TRCN0000372275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144966.4-2070s21c1
DNA Barcode:
TTCGTGGTCTTCCGGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FREM1 (158326)
Gene Description:
FRAS1 related extracellular matrix 1
Transcript:
RefSeq NM_144966.4 (NON-CURRENT)
Match location:
Position 2070 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGTGGTCTTCCGGATATTT
Hairpin Sequence:
5'-CCGG-TTCGTGGTCTTCCGGATATTT-CTCGAG-AAATATCCGGAAGACCACGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGTGGTCTTCCGGATATTTCTCGAGAAATATCCGGAAGACCACGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGTGGTCTTCCGGATATTTCTCGAGAAATATCCGGAAGACCACGAA-3'

Other clones with same target sequence:

(none)