Construct: shRNA TRCN0000372418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000415.2-579s21c1
DNA Barcode:
ACTAACTAAGGTCCCATAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IAPP (3375)
Gene Description:
islet amyloid polypeptide
Transcript:
RefSeq NM_000415.2 (NON-CURRENT)
Match location:
Position 579 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTAACTAAGGTCCCATAATA
Hairpin Sequence:
5'-CCGG-ACTAACTAAGGTCCCATAATA-CTCGAG-TATTATGGGACCTTAGTTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTAACTAAGGTCCCATAATACTCGAGTATTATGGGACCTTAGTTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTAACTAAGGTCCCATAATACTCGAGTATTATGGGACCTTAGTTAGT-3'

Other clones with same target sequence:

(none)