Construct: shRNA TRCN0000372534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000585.3-1208s21c1
DNA Barcode:
TAAGGGTGATAGTCAAATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL15 (3600)
Gene Description:
interleukin 15
Transcript:
RefSeq NM_000585.3 (NON-CURRENT)
Match location:
Position 1208 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGGGTGATAGTCAAATTAT
Hairpin Sequence:
5'-CCGG-TAAGGGTGATAGTCAAATTAT-CTCGAG-ATAATTTGACTATCACCCTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGGGTGATAGTCAAATTATCTCGAGATAATTTGACTATCACCCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGGGTGATAGTCAAATTATCTCGAGATAATTTGACTATCACCCTTA-3'

Other clones with same target sequence:

(none)