Construct: shRNA TRCN0000372724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000418.2-2748s21c1
DNA Barcode:
CAGATGAGGACTAGGGCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL4R (3566)
Gene Description:
interleukin 4 receptor
Transcript:
RefSeq NM_000418.2 (NON-CURRENT)
Match location:
Position 2748 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGAGGACTAGGGCTTAT
Hairpin Sequence:
5'-CCGG-CAGATGAGGACTAGGGCTTAT-CTCGAG-ATAAGCCCTAGTCCTCATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGAGGACTAGGGCTTATCTCGAGATAAGCCCTAGTCCTCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGAGGACTAGGGCTTATCTCGAGATAAGCCCTAGTCCTCATCTG-3'

Other clones with same target sequence:

(none)