Construct: shRNA TRCN0000372899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015274.1-1601s21c1
DNA Barcode:
CTGCGTATGACCTGCTTATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAN2B2 (23324)
Gene Description:
mannosidase alpha class 2B member 2
Transcript:
RefSeq NM_015274.1 (NON-CURRENT)
Match location:
Position 1601 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCGTATGACCTGCTTATTC
Hairpin Sequence:
5'-CCGG-CTGCGTATGACCTGCTTATTC-CTCGAG-GAATAAGCAGGTCATACGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCGTATGACCTGCTTATTCCTCGAGGAATAAGCAGGTCATACGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCGTATGACCTGCTTATTCCTCGAGGAATAAGCAGGTCATACGCAG-3'

Other clones with same target sequence:

(none)