Construct: shRNA TRCN0000372977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012214.2-1436s21c1
DNA Barcode:
ACTCACGGATAAAGATTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGAT4A (11320)
Gene Description:
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
Transcript:
RefSeq NM_012214.2 (NON-CURRENT)
Match location:
Position 1436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000372977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCACGGATAAAGATTATAT
Hairpin Sequence:
5'-CCGG-ACTCACGGATAAAGATTATAT-CTCGAG-ATATAATCTTTATCCGTGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCACGGATAAAGATTATATCTCGAGATATAATCTTTATCCGTGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCACGGATAAAGATTATATCTCGAGATATAATCTTTATCCGTGAGT-3'

Other clones with same target sequence:

(none)