Construct: shRNA TRCN0000373092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006157.3-1508s21c1
DNA Barcode:
TCAACCTTCCTGGGTTATATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NELL1 (4745)
Gene Description:
neural EGFL like 1
Transcript:
RefSeq NM_006157.3 (NON-CURRENT)
Match location:
Position 1508 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAACCTTCCTGGGTTATATC
Hairpin Sequence:
5'-CCGG-TCAACCTTCCTGGGTTATATC-CTCGAG-GATATAACCCAGGAAGGTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAACCTTCCTGGGTTATATCCTCGAGGATATAACCCAGGAAGGTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAACCTTCCTGGGTTATATCCTCGAGGATATAACCCAGGAAGGTTGA-3'

Other clones with same target sequence:

(none)