Construct: shRNA TRCN0000373136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002594.2-401s21c1
DNA Barcode:
ACCGGTCTTCACGAATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCSK2 (5126)
Gene Description:
proprotein convertase subtilisin/kexin type 2
Transcript:
RefSeq NM_002594.2 (NON-CURRENT)
Match location:
Position 401 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGGTCTTCACGAATCATTT
Hairpin Sequence:
5'-CCGG-ACCGGTCTTCACGAATCATTT-CTCGAG-AAATGATTCGTGAAGACCGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGGTCTTCACGAATCATTTCTCGAGAAATGATTCGTGAAGACCGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGGTCTTCACGAATCATTTCTCGAGAAATGATTCGTGAAGACCGGT-3'

Other clones with same target sequence:

(none)