Construct: shRNA TRCN0000373143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000442.3-1347s21c1
DNA Barcode:
GTCGGACAGTGGGACGTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PECAM1 (5175)
Gene Description:
platelet and endothelial cell adhesion molecule 1
Transcript:
RefSeq NM_000442.3 (NON-CURRENT)
Match location:
Position 1347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGGACAGTGGGACGTATAT
Hairpin Sequence:
5'-CCGG-GTCGGACAGTGGGACGTATAT-CTCGAG-ATATACGTCCCACTGTCCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGGACAGTGGGACGTATATCTCGAGATATACGTCCCACTGTCCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGGACAGTGGGACGTATATCTCGAGATATACGTCCCACTGTCCGAC-3'

Other clones with same target sequence:

(none)