Construct: shRNA TRCN0000373154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007226.2-809s21c1
DNA Barcode:
GTGGAAATGAGGGATACATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NXPH2 (11249)
Gene Description:
neurexophilin 2
Transcript:
RefSeq NM_007226.2 (NON-CURRENT)
Match location:
Position 809 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAAATGAGGGATACATAT
Hairpin Sequence:
5'-CCGG-GTGGAAATGAGGGATACATAT-CTCGAG-ATATGTATCCCTCATTTCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAAATGAGGGATACATATCTCGAGATATGTATCCCTCATTTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAAATGAGGGATACATATCTCGAGATATGTATCCCTCATTTCCAC-3'

Other clones with same target sequence:

(none)