Construct: shRNA TRCN0000373209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003561.1-468s21c1
DNA Barcode:
ATCATGCTGCTCCTGCTACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLA2G10 (8399)
Gene Description:
phospholipase A2 group X
Transcript:
RefSeq NM_003561.1 (NON-CURRENT)
Match location:
Position 468 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATGCTGCTCCTGCTACTG
Hairpin Sequence:
5'-CCGG-ATCATGCTGCTCCTGCTACTG-CTCGAG-CAGTAGCAGGAGCAGCATGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATGCTGCTCCTGCTACTGCTCGAGCAGTAGCAGGAGCAGCATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATGCTGCTCCTGCTACTGCTCGAGCAGTAGCAGGAGCAGCATGAT-3'

Other clones with same target sequence:

(none)