Construct: shRNA TRCN0000373263

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006905.2-1723s21c1
DNA Barcode:
TAATCTATCTACAGCTTATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSG1 (5669)
Gene Description:
pregnancy specific beta-1-glycoprotein 1
Transcript:
RefSeq NM_006905.2 (NON-CURRENT)
Match location:
Position 1723 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373263)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATCTATCTACAGCTTATAG
Hairpin Sequence:
5'-CCGG-TAATCTATCTACAGCTTATAG-CTCGAG-CTATAAGCTGTAGATAGATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATCTATCTACAGCTTATAGCTCGAGCTATAAGCTGTAGATAGATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATCTATCTACAGCTTATAGCTCGAGCTATAAGCTGTAGATAGATTA-3'

Other clones with same target sequence:

(none)