Construct: shRNA TRCN0000373314

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014636.1-819s21c1
DNA Barcode:
CAAGCGGACACGGGAATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGPS1 (9649)
Gene Description:
Ral GEF with PH domain and SH3 binding motif 1
Transcript:
RefSeq NM_014636.1 (NON-CURRENT)
Match location:
Position 819 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373314)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCGGACACGGGAATATAT
Hairpin Sequence:
5'-CCGG-CAAGCGGACACGGGAATATAT-CTCGAG-ATATATTCCCGTGTCCGCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCGGACACGGGAATATATCTCGAGATATATTCCCGTGTCCGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCGGACACGGGAATATATCTCGAGATATATTCCCGTGTCCGCTTG-3'

Other clones with same target sequence:

(none)