Construct: shRNA TRCN0000373325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021016.3-1629s21c1
DNA Barcode:
GGCTGATACTTCATGAAATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSG3 (5671)
Gene Description:
pregnancy specific beta-1-glycoprotein 3
Transcript:
RefSeq NM_021016.3 (NON-CURRENT)
Match location:
Position 1629 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTGATACTTCATGAAATTC
Hairpin Sequence:
5'-CCGG-GGCTGATACTTCATGAAATTC-CTCGAG-GAATTTCATGAAGTATCAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTGATACTTCATGAAATTCCTCGAGGAATTTCATGAAGTATCAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTGATACTTCATGAAATTCCTCGAGGAATTTCATGAAGTATCAGCC-3'

Other clones with same target sequence:

(none)