Construct: shRNA TRCN0000373609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003037.2-1669s21c1
DNA Barcode:
CTTGGCTGGAACTGGATAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLAMF1 (6504)
Gene Description:
signaling lymphocytic activation molecule family member 1
Transcript:
RefSeq NM_003037.2 (NON-CURRENT)
Match location:
Position 1669 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGCTGGAACTGGATAATA
Hairpin Sequence:
5'-CCGG-CTTGGCTGGAACTGGATAATA-CTCGAG-TATTATCCAGTTCCAGCCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGCTGGAACTGGATAATACTCGAGTATTATCCAGTTCCAGCCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGCTGGAACTGGATAATACTCGAGTATTATCCAGTTCCAGCCAAG-3'

Other clones with same target sequence:

(none)