Construct: shRNA TRCN0000373746

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015926.4-251s21c1
DNA Barcode:
ACGTCACTGTGGCCTACAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEX264 (51368)
Gene Description:
testis expressed 264, ER-phagy receptor
Transcript:
RefSeq NM_015926.4 (NON-CURRENT)
Match location:
Position 251 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373746)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCACTGTGGCCTACAAGT
Hairpin Sequence:
5'-CCGG-ACGTCACTGTGGCCTACAAGT-CTCGAG-ACTTGTAGGCCACAGTGACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCACTGTGGCCTACAAGTCTCGAGACTTGTAGGCCACAGTGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCACTGTGGCCTACAAGTCTCGAGACTTGTAGGCCACAGTGACGT-3'

Other clones with same target sequence:

(none)