Construct: shRNA TRCN0000373860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182546.2-833s21c1
DNA Barcode:
AGGCCTATCTGAAAGTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VSTM2A (222008)
Gene Description:
V-set and transmembrane domain containing 2A
Transcript:
RefSeq NM_182546.2 (NON-CURRENT)
Match location:
Position 833 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCCTATCTGAAAGTCAATG
Hairpin Sequence:
5'-CCGG-AGGCCTATCTGAAAGTCAATG-CTCGAG-CATTGACTTTCAGATAGGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCCTATCTGAAAGTCAATGCTCGAGCATTGACTTTCAGATAGGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCCTATCTGAAAGTCAATGCTCGAGCATTGACTTTCAGATAGGCCT-3'

Other clones with same target sequence:

  1. TRCN0000435368