Construct: shRNA TRCN0000373991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080428.2-1309s21c1
DNA Barcode:
ACTTCTACTGTACGGTGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxw7 (50754)
Gene Description:
F-box and WD-40 domain protein 7
Transcript:
RefSeq NM_080428.2 (NON-CURRENT)
Match location:
Position 1309 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTCTACTGTACGGTGTATG
Hairpin Sequence:
5'-CCGG-ACTTCTACTGTACGGTGTATG-CTCGAG-CATACACCGTACAGTAGAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTCTACTGTACGGTGTATGCTCGAGCATACACCGTACAGTAGAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTCTACTGTACGGTGTATGCTCGAGCATACACCGTACAGTAGAAGT-3'

Other clones with same target sequence:

(none)