Construct: shRNA TRCN0000374312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021463.3-1554s21c1
DNA Barcode:
GGTAGCTCAATGCAGTAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prps1 (19139)
Gene Description:
phosphoribosyl pyrophosphate synthetase 1
Transcript:
RefSeq NM_021463.3 (NON-CURRENT)
Match location:
Position 1554 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTAGCTCAATGCAGTAATTA
Hairpin Sequence:
5'-CCGG-GGTAGCTCAATGCAGTAATTA-CTCGAG-TAATTACTGCATTGAGCTACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTAGCTCAATGCAGTAATTACTCGAGTAATTACTGCATTGAGCTACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTAGCTCAATGCAGTAATTACTCGAGTAATTACTGCATTGAGCTACC-3'

Other clones with same target sequence:

(none)