Construct: shRNA TRCN0000374337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145124.2-1005s21c1
DNA Barcode:
AGGGCACACGTACCAACTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mib2 (76580)
Gene Description:
mindbomb E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_145124.2 (NON-CURRENT)
Match location:
Position 1005 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGCACACGTACCAACTATC
Hairpin Sequence:
5'-CCGG-AGGGCACACGTACCAACTATC-CTCGAG-GATAGTTGGTACGTGTGCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGCACACGTACCAACTATCCTCGAGGATAGTTGGTACGTGTGCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGCACACGTACCAACTATCCTCGAGGATAGTTGGTACGTGTGCCCT-3'

Other clones with same target sequence:

(none)