Construct: shRNA TRCN0000374405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198894.1-3077s21c1
DNA Barcode:
TTTCTCCCAGCACTCACTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abr (109934)
Gene Description:
active BCR-related gene
Transcript:
RefSeq NM_198894.1 (NON-CURRENT)
Match location:
Position 3077 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCTCCCAGCACTCACTTTC
Hairpin Sequence:
5'-CCGG-TTTCTCCCAGCACTCACTTTC-CTCGAG-GAAAGTGAGTGCTGGGAGAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCTCCCAGCACTCACTTTCCTCGAGGAAAGTGAGTGCTGGGAGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCTCCCAGCACTCACTTTCCTCGAGGAAAGTGAGTGCTGGGAGAAA-3'

Other clones with same target sequence:

(none)