Construct: shRNA TRCN0000374427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019501.3-283s21c1
DNA Barcode:
CCCAACTATGCATAGTATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdss1 (56075)
Gene Description:
prenyl (solanesyl) diphosphate synthase, subunit 1
Transcript:
RefSeq NM_019501.3 (CURRENT)
Match location:
Position 283 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACTATGCATAGTATATC
Hairpin Sequence:
5'-CCGG-CCCAACTATGCATAGTATATC-CTCGAG-GATATACTATGCATAGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACTATGCATAGTATATCCTCGAGGATATACTATGCATAGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACTATGCATAGTATATCCTCGAGGATATACTATGCATAGTTGGG-3'

Other clones with same target sequence:

(none)