Construct: shRNA TRCN0000374468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023912.2-291s21c1
DNA Barcode:
CCCAACATCCTGGCCTATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scyl1 (78891)
Gene Description:
SCY1-like 1 (S. cerevisiae)
Transcript:
RefSeq NM_023912.2 (CURRENT)
Match location:
Position 291 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACATCCTGGCCTATATC
Hairpin Sequence:
5'-CCGG-CCCAACATCCTGGCCTATATC-CTCGAG-GATATAGGCCAGGATGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACATCCTGGCCTATATCCTCGAGGATATAGGCCAGGATGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACATCCTGGCCTATATCCTCGAGGATATAGGCCAGGATGTTGGG-3'

Other clones with same target sequence:

(none)