Construct: shRNA TRCN0000374580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011500.2-2259s21c1
DNA Barcode:
CAATACGCTTATGGAATTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Strn (268980)
Gene Description:
striatin, calmodulin binding protein
Transcript:
RefSeq NM_011500.2 (CURRENT)
Match location:
Position 2259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATACGCTTATGGAATTTAG
Hairpin Sequence:
5'-CCGG-CAATACGCTTATGGAATTTAG-CTCGAG-CTAAATTCCATAAGCGTATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATACGCTTATGGAATTTAGCTCGAGCTAAATTCCATAAGCGTATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATACGCTTATGGAATTTAGCTCGAGCTAAATTCCATAAGCGTATTG-3'

Other clones with same target sequence:

(none)