Construct: shRNA TRCN0000374857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025295.3-747s21c1
DNA Barcode:
CACTTGCTTTGACATCCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Btd (26363)
Gene Description:
biotinidase
Transcript:
RefSeq NM_025295.3 (NON-CURRENT)
Match location:
Position 747 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTGCTTTGACATCCTATT
Hairpin Sequence:
5'-CCGG-CACTTGCTTTGACATCCTATT-CTCGAG-AATAGGATGTCAAAGCAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTGCTTTGACATCCTATTCTCGAGAATAGGATGTCAAAGCAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTGCTTTGACATCCTATTCTCGAGAATAGGATGTCAAAGCAAGTG-3'

Other clones with same target sequence:

(none)