Construct: shRNA TRCN0000375017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007984.2-1220s21c1
DNA Barcode:
TTCCTCATGAAGCTGATTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fscn1 (14086)
Gene Description:
fascin actin-bundling protein 1
Transcript:
RefSeq NM_007984.2 (CURRENT)
Match location:
Position 1220 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCTCATGAAGCTGATTAAC
Hairpin Sequence:
5'-CCGG-TTCCTCATGAAGCTGATTAAC-CTCGAG-GTTAATCAGCTTCATGAGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCTCATGAAGCTGATTAACCTCGAGGTTAATCAGCTTCATGAGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCTCATGAAGCTGATTAACCTCGAGGTTAATCAGCTTCATGAGGAA-3'

Other clones with same target sequence:

(none)