Construct: shRNA TRCN0000375037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016715.4-414s21c1
DNA Barcode:
GCCATGGTGTTTAAGGCTAGG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crlf2 (57914)
Gene Description:
cytokine receptor-like factor 2
Transcript:
RefSeq NM_016715.4 (CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGGTGTTTAAGGCTAGG
Hairpin Sequence:
5'-CCGG-GCCATGGTGTTTAAGGCTAGG-CTCGAG-CCTAGCCTTAAACACCATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGGTGTTTAAGGCTAGGCTCGAGCCTAGCCTTAAACACCATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGGTGTTTAAGGCTAGGCTCGAGCCTAGCCTTAAACACCATGGC-3'

Other clones with same target sequence:

(none)