Construct: shRNA TRCN0000375065

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013885.2-621s21c1
DNA Barcode:
ACTGGATGAGTACCTCAACTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clic4 (29876)
Gene Description:
chloride intracellular channel 4 (mitochondrial)
Transcript:
RefSeq NM_013885.2 (CURRENT)
Match location:
Position 621 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375065)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGATGAGTACCTCAACTC
Hairpin Sequence:
5'-CCGG-ACTGGATGAGTACCTCAACTC-CTCGAG-GAGTTGAGGTACTCATCCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGATGAGTACCTCAACTCCTCGAGGAGTTGAGGTACTCATCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGATGAGTACCTCAACTCCTCGAGGAGTTGAGGTACTCATCCAGT-3'

Other clones with same target sequence:

(none)